Journal: Nature Communications

Article Title: Schwann cells regulate tumor cells and cancer-associated fibroblasts in the pancreatic ductal adenocarcinoma microenvironment

doi: 10.1038/s41467-023-40314-w

Figure Lengend Snippet: a The relative mRNA levels of iCAF and myCAF markers in CAF-2 cultured with RSC96 CM and control. b The protein level of α-SMA in CAF-2 cultured with 0, 20, or 100 μg/ml RSC96 CM. c The fold change of IL-6 in CAF-2 cultured with RSC96 CM for 0, 1, 6, or 24 h was evaluated by ELISA. d Representative images and quantification of IF with anti-α-SMA in CAF-2 cultured with RSC96 CM and control. Scale bar, 100 µm. Images are representative of three biologically independent experiments with CAF-2 cells with similar results. e QuSAGE scores of control and SC-CM associated signature in perineural tier 1–4 and other regions in the neuro-stroma niche. The signatures were defined by the bulk RNA-seq of CAFs with SC-CM treatment. f Schematic diagram of ex vivo assay. g The relative mRNA levels of iCAF and myCAF markers in PDAC tissue cultured with RSC96 CM and control. h , i Representative images ( h ) and quantification ( i ) of IF with anti-α-SMA and IL-6 in PDAC tissue cultured with RSC96 CM and control. Scale bar, 20 µm. j Representative images and quantification of IL-1α in PDAC tissues. Scale bar, 20 µm. h , j Images are representative of five PDAC samples with similar results. k The relative mRNA levels of iCAF and myCAF markers in CAF-2 treated with IL-1α or RSC96 CM. l The relative mRNA levels of iCAF and myCAF markers in CAF-2 cultured by RSC96 CM pretreated with neutralization antibodies against IL-1α or IgG control. m Schematic diagram of how Schwann cells shape tumor cells and CAFs in the neuro-stroma niche. n Schematic diagram of the in vivo orthotopic injection. A cell mixture of CAFs, CFPAC-1, and sNF96.2 cells was injected into mice pancreas, then the mice subjected to MDK inhibitor (iMDK) and interleukin-1 receptor antagonist (IL-1RA) treatment (INHs, inhibitors) or not ( n = 5). o – q Photograph ( o ) and quantification ( p , q ) of orthotopic tumors of mice. a , c , d , g , k . l Data were the mean ± s.d. of n = 3 independent experiments. i , p , q Data were the mean ± s.d. of n = 5 independent experiments. f , m , n Created with BioRender.com. Statistical analysis: unpaired two-sided t -test ( a , c , d , g , i , k , l , p , q ); paired two-sided t -test ( j ). Source data are provided as a Source Data file.

Article Snippet: After 14 days, mice were divided into control or inhibitors groups, the latter were treated with iMDK (9 mg/kg, MCE, HY-110171A) and IL-1RA (25 mg/kg, MCE, AMG-719) by intraperitoneal injection every 48 h. For the co-injection model in Fig. , 2 × 10 6 Panc-1 or CFPAC-1 and 2 × 10 6 CAF-2 (w/ or w/o RSC96 CM/IL-1RA (1 μg/ml) treatment) were injected into the pancreas.

Techniques: Cell Culture, Control, Enzyme-linked Immunosorbent Assay, RNA Sequencing Assay, Ex Vivo, Neutralization, In Vivo, Injection

Journal: Nature Communications

Article Title: Schwann cells regulate tumor cells and cancer-associated fibroblasts in the pancreatic ductal adenocarcinoma microenvironment

doi: 10.1038/s41467-023-40314-w

Figure Lengend Snippet: a ‒ c The migration capabilities of Panc-1 cells were enhanced by the CM of activated CAFs. CAFs were directly co-cultured ( a ) or Transwell co-cultured with ( b ) Schwann cells, or cultured with Schwann cells CM ( c ), respectively. Direct co-culture ( a ): CM of CAFs, RSC96, and cell mixture of CAFs and RSC96 were harvested. Transwell co-culture ( b ): CAFs and RSC96 cells were co-cultured via Transwell chambers for 48 h, and CAFs was washed by PBS three times, then CAFs were cultured with fresh medium for another 48 h, at last, CAFs CM was harvested. Schwann cell CM ( c ): CAFs was cultured with Schwann cell CM for 48 h and the removal of SC-CM was followed by three rinses with PBS, and then CAFs were cultured with fresh medium for another 48 h, at last, CAFs CM was harvested. After 48 h culture with CAFs CM, Panc-1 cells were harvested for migration assay. d The protein levels of E-cadherin, Vimentin, Fibronectin, and β-catenin in Panc-1 cells treated with CM of CAFs, which were cultured with or without (w/ or w/o) RSC96 CM. Data were representative of n = 3 independent experiments. e Schematic diagram of the in vivo orthotopic injection. A cell mixture of CAFs (w/ or w/o RSC96 CM/IL-1RA treatment) and tumor cells was injected into mice pancreas ( n = 5). f , g Bioluminescence photograph ( f ) and quantification ( g ) of orthotopic tumors of mice injected with a cell mixture of Panc-1 cells and CAFs. h Schematic diagram of in vivo liver metastatic model. Panc-1 ( i , j ) or CFPAC-1( k , l ) were pretreated with ctrl medium (G1), SC-CM(G2), SC-CM+iMDK(G3), ctrl-CAF-CM(G4), SC-induced CAF-CM(G5), or SC + IL-1RA-induced CAF-CM (G6), and then injected via spleen ( n = 5). i – l Representative photograph ( i , k ) and quantification ( j , l ) of Panc-1 ( i , j ) or CFPAC-1 ( k , l ) liver metastatic tumors. a , c (right), Data were the mean ± s.d. of n = 4 independent experiments. b , c (left), g , j , l Data were the mean ± s.d. of n = 5 independent experiments. a – c , e , h Created with BioRender.com. Statistical analysis: unpaired two-sided t -test ( a – c , j , l ); two-way ANOVA ( g ). Source data are provided as a Source Data file.

Article Snippet: After 14 days, mice were divided into control or inhibitors groups, the latter were treated with iMDK (9 mg/kg, MCE, HY-110171A) and IL-1RA (25 mg/kg, MCE, AMG-719) by intraperitoneal injection every 48 h. For the co-injection model in Fig. , 2 × 10 6 Panc-1 or CFPAC-1 and 2 × 10 6 CAF-2 (w/ or w/o RSC96 CM/IL-1RA (1 μg/ml) treatment) were injected into the pancreas.

Techniques: Migration, Cell Culture, Co-Culture Assay, In Vivo, Injection

Journal: Human Brain Mapping

Article Title: Dyconnmap: Dynamic connectome mapping—A neuroimaging python module

doi: 10.1002/hbm.25589

Figure Lengend Snippet: Methods available in the ‘graph’ submodule

Article Snippet: Ipsen‐Mikhailov distance (IMD) , Given two graphs, this method quantifies their difference by comparing their spectral densities. This spectral density is computed as the sum of Lorentz distributions (Ipsen, ; Donnat & Holmes ). .

Techniques: Diffusion-based Assay, Transmission Assay